Phusion DNA Polymerase brings together a novel Pyrococcus-like enzyme with a processivity-enhancing domain and generates PCR products with accuracy and speed previously unattainable with a single enzyme, even on your most difficult templates. Additionally, Phusion DNA Polymerase is capable of amplifying long templates.

What is Phusion High-Fidelity DNA polymerase?

Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.

Does phusion create blunt ends?

Phusion DNA Polymerases generate blunt end products; therefore, blunt end cloning is recommended. Any remaining Phusion DNA Polymerase will degrade the A overhangs, thus creating blunt ends again.

What is the difference between phusion and Taq polymerase?

Phusion is a DNA polymerase isolated from Pyrococcus furiosus and is mainly used in cloning experiments to increase the fidelity. Taq DNA Polymerase is the standard DNA polymerase used in the Polymerase Chain Reaction (PCR), and it is isolated from the thermostable bacterium, Thermus aquaticus.

What are the 3 main functions of DNA polymerase?

The function of DNA polymerase is to replicate, proofread and repair DNA. Several DNA polymerases exist, but DNA polymerase I , or Pol I, and DNA polymerase III , or Pol III , are the main ones involved in DNA replication. DNA polymerase adds nucleotide bases only when an RNA primer , a short piece of RNA ,…

How does Taq polymerase work?

Taq polymerase is an enzyme that is used by scientists in the polymerase chain reaction because it is stable at high temperatures. Large amounts of a sample of DNA are necessary for molecular and genetic analyses, and the polymerase chain reaction is used to amplify very small amounts of DNA.

What are the different types of DNA polymerase?

DNA polymerases. E. Coli. Three types of DNA polymerases exist in E. coli: I, II and III. The DNA polymerase I is used to fill the gap between DNA fragments of the lagging strand. It is also the major enzyme for gap filling during DNA repair. The DNA polymerase II is encoded by the PolB gene, which is involved in the SOS response to DNA damage.

What is DNA polymerase and its function?

The main function of DNA polymerase is to make DNA from nucleotides, the building blocks of DNA. There are several forms of DNA polymerase that play a role in DNA replication and they usually work in pairs to copy one molecule of double-stranded DNA into two new double stranded DNA molecules. This process is called semi-conservative replication.